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ABSTRACT Purpose: To assess the effects of the preoperative application of artificial tears combined with recombinant bovine basic fibroblast growth factor on the ocular surface function and inflammatory factor levels after operation in cataract patients complicated with dry eyes. Methods: A total of 118 cataract patients (118 eyes) complicated with dry eyes treated from February 2019 to February2020 were assigned to control and observation groups (n=59 eyes/group) using a random number table. One week before the operation, the control group was administered 0.1% sodium hyaluronate eye drops (artificial tears), based on which the observation group received Beifushu eye drops (recombinant bovine basic fibroblast growth factor), both 6 times daily for 1 week. A comparison was made between the scores of clinical symptoms and the indices of ocular surface function, inflammatory factors in tears, and oxidative stress indices before and after the operation. The ocular surface function was evaluated by an ocular surface disease index questionnaire, tear film breakup-time assay, Schirmer's I test, and corneal fluorescein stain test. The inflammatory factors in tears were measured. Results: No significant differences were noted in the general data and clinical symptom score, ocular surface disease index, tear film breakup-time, Schirmer's I test score, fluorescein stain score, interleukin-6, tumor necrosis factor-alpha, malondialdehyde, superoxide dismutase, lipid peroxide, and total antioxidant capacity before treatment between the 2 groups (p>0.05). After treatment, the clinical symptom score, ocular surface disease index, fluorescein stain score, tumor necrosis factor-alpha, interleukin-6, malondial-dehyde and lipid peroxide declined significantly, and tear film breakup-time, Schirmer's I test score, superoxide dismutase, and total antioxidant capacity increased in both the groups. The improvements in the clinical symptom score as well as in the indices of ocular surface function, inflammatory factors, and oxidative stress were more prominent in the observation group than in the control group (p<0.05). Conclusions: Artificial tears combined with recombinant bovine basic fibroblast growth factor before operation. significantly improved the ocular surface function, reduced inflammatory factors in tears, and alleviated dry eye symptoms after operation in cataract patients.
RESUMO Objetivo: Avaliar os efeitos da aplicação pré-operatória de lágrimas artificiais combinadas com o fator de crescimento de fibroblastos básicos bovinos recombinantes na função da superfície ocular e níveis de fator inflamatório após cirurgia em pacientes com catarata complicada com olhos secos. Métodos: Um total de 118 pacientes com catarata complicada com olhos secos (118 olhos), tratados entre fevereiro de 2019 e fevereiro de 2020, foram divididos em grupos de controle e de observação (n=59, 59 olhos) usando uma tabela de números aleatórios. Uma semana antes da cirurgia, o grupo controle recebeu colírio de hialuronato de sódio a 0,1% (lágrimas artificiais), enquanto o grupo de observação recebeu colírio Beifushu (fator de crescimento de fibroblastos básicos bovinos recombinantes), ambos, seis vezes ao dia, por uma semana. Antes do tratamento e um mês após a cirurgia, os escores de sintomas clínicos, índices de função da superfície ocular, níveis de fatores inflamatórios nas lágrimas e índices de estresse oxidativo foram comparados. A função da superfície ocular foi avaliada pelo questionário do índice de doença da superfície ocular, ensaio de tempo de ruptura do filme lacrimal, teste I de Schirmer e teste de coloração por fluoresceína da córnea. Os níveis de fatores inflamatórios nas lágrimas foram medidos. Resultados: Não houve diferenças significativas nos dados gerais e no escore de sintomas clínicos, índice de doença da superfície ocular, tempo de ruptura do filme lacrimal, escore do teste I de Schirmer, pontuação do teste de coloração por fluoresceína da córnea, interleucina-6, fator de necrose tumoral alfa, malondialdeído, superóxido dismutase, peróxido lipídico e capacidade antioxidante total antes do tratamento entre os dois grupos (p>0,05). Após o tratamento, o escore de sintomas clínicos, índice de doença da superfície ocular, escore do teste de coloração por fluoresceína da córnea, fator de necrose tumoral alfa, interleucina-6, malondialdeído e peróxido lipídico diminuíram significativamente, e o tempo de ruptura do filme lacrimal, escore do teste I de Schirmer, superóxido dismutase e a capacidade antioxidante total aumentou em ambos os grupos. As melhorias no escore de sintomas clínicos, bem como os índices de função da superfície ocular, fatores inflamatórios e estresse oxidativo foram mais proeminentes no grupo de observação do que no grupo controle (p<0,05). Conclusões: Lágrimas artificiais combinadas com fator de crescimento de fibroblastos básicos recombinantes antes da cirurgia melhoram notavelmente a função da superfície ocular, diminuem os níveis de fatores inflamatórios nas lágrimas e aliviam os sintomas de olho seco após a cirurgia em pacientes com catarata complicada com olhos secos.
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Purpose: To evaluate dry eyes in children with vernal kerato?conjunctivitis (VKC) and correlate it with symptoms, clinical findings, and ocular surface analysis (OSA) parameters. Methods: Children with clinically diagnosed VKC underwent complete ophthalmological examination, Schirmer’s testing, modified ocular surface disease index (OSDI) scoring, Bonini grading, fluorescein tear?film break?up time (TBUT), VKC – Collaborative Longitudinal Evaluation of Keratoconus (CLEK) scoring, and OSA. Children with a TBUT of < 10 s were defined to have dry eyes. The above?mentioned parameters were compared between dry eye and non?dry eye VKC children. Results: The mean age of the 87 children included in the study was 9.1 ± 2.9 years. Dry eyes were seen in 60.9% [95% confidence interval (CI); 51% to 71%]. The mean TBUT was 13.4 ± 3.8 and 5.9 ± 1.9 s in non?dry and dry eye groups, respectively (P < 0.001). The mean value of Schirmer’s test was 25.9 ± 9.8 and 20.8 ± 8.6 mm in the non?dry and dry eye groups, respectively (P = 0.01). The two groups did not differ in their OSDI scores, Bonini grading, and CLEK scores. The OSA parameter of non?invasive break?up time (NIBUT) was 8.3 ± 3.2 s in non?dry eye group and 6.4 ± 2.9 s in dry eye group, P = 0.008. The lower lid Meibomian gland (MG) loss was 7.4% in non?dry eye group and 12.2% in dry eye group, P = 0.028. Other OSA parameters did not differ significantly among the two groups. Conclusion: Dry eyes are seen in two?thirds of pediatric VKC. Evaluation of dry eyes should be incorporated in their clinical evaluation. Among OSA parameters, NIBUT and lower lid MG loss are associated with dry eyes in pediatric VKC patients.
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Purpose: Ocular manifestations in psoriasis are due to direct eye involvement with psoriatic plaques or psoriasis?related, immune?mediated inflammatory processes. The commonly reported pathologies are blepharitis, conjunctivitis, keratitis, dry eyes, and uveitis. Limited data is available on the ocular findings in psoriasis patients in India. In this study, we evaluated various ocular changes associated with moderate-to-severe psoriasis. Methods: In this prospective cohort study, treatment?naive psoriasis patients with Psoriasis Area Severity Index (PASI) score of more than 10 were included. The Ocular Surface Disease Index (OSDI) score, Schirmer’s score, tear film breakup time (TBUT), corneal and conjunctival staining score, and meibomian gland dysfunction score were noted. All these parameters were re?evaluated at 8 weeks of follow?up after systemic treatment. Results: Sixty-eight patients were enrolled in the study. The most common ocular pathologies observed in this study were tarsal hyperemia and anterior blepharitis in 128 (94.1%) and 64 (47%) eyes, respectively. Mild, moderate, and severe dry eyes were seen in 26 (19.1%), 14 (10.2%), and 34 (25%) eyes, respectively. Thirty?nine (57.3%) patients complained of significant difficulty watching television or digital screen. In 21 patients evaluated on follow?up at 8 weeks, cornea and conjunctiva’s ocular surface staining score increased and TBUT decreased significantly. Conclusion: The most common ocular pathologies observed in this study were anterior blepharitis and moderate dry eye, which significantly affected most patients’ daily routines. Screening patients with greater severity of psoriasis would help in early management of such problems.
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@#AIM:To investigate the dry eye symptoms and the stability changes of tear film after correction of myopia by laser-assisted <i>in situ</i> keratomileusis(LASIK)and laser epithelial keratomileusis(LASEK)with the use of 0.1% sodium hyaluronate(HA).<p>METHODS:A total of 230 myopia patients(460 eyes)were divided into LASIK group and LASEK group from February 2017 to December 2017. Each operation group was randomly assigned to 0.1% HA treatment subgroup and HA-free subgroup. Both subgroups were instructed to apply gatifloxacin(0.3%)and loteprednol(0.5%)after the corneal refractive surgery. The changes of dry eye symptom score, corneal perceptual reaction, corneal fluorescein staining(FL), basic Schirmer Ⅰ test(SⅠt)and tear film break-up time(BUT)were observed in different time points.<p>RESULTS:There were significant differences in FL, dry eye symptom score and corneal perceptual response between the 0.1% HA treatment group and HA-free group at 1wk, 1, 3 and 6mo after operation both in LASIK and LASEK patients(<i>P</i><0.05). BUT after operation in each group was significantly deceased at 1wk compared with preoperative level. Other observation time points after surgery of BUT and each postoperative level of SⅠt were still within the normal range although lower than the preoperative levels. The subjective symptoms of dry eyes, tear film stability and corneal surface perception of LASEK patients were better than LASIK patients especially at 1wk, 1 and 3mo after surgery.<p>CONCLUSION:These two kinds of refractive surgery may cause different degrees of dry eye symptoms. These symptoms in the LASEK group were lighter than that in the LASIK group in the early postoperative period. The early adequate use of preservative-free 0.1% HA could effectively promote the corneal repair and be greatly helpful for postoperative dry eye symptoms.
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@#Meibomian gland dysfunction(MGD)is a general term for various abnormal meibomian glands. Meibomian gland secretes meibomian ester to moisten the eye surface and maintain the stability of tear film. In this article, the etiology, pathology and related treatment of meibomian gland dysfunction were reviewed by referring to related literatures.
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@#AIM: To analyze the correlation between tear film lipid layer thickness(LLT)and macular microvascular parameters in diabetic retinopathy. <p>METHODS: Totally 60 eyes of type 2 diabetes patients with non-proliferative stage(NPDR group)and 60 eyes of proliferative stage(PDR group)with diabetic retinopathy diagnosed in our hospital from 2018-01/12 were selected, and 60 eyes of healthy volunteers with appropriate age and gender were selected as the normal control group. The tear film lipid layer thickness(LLT)was examined by Lipiview eye surface interferometer, while the foveal avascular zone(FAZ), superficial capillary layer(SCL)vessel density and deep capillary layer(DCL)vessel density were measured by optical coherence tomography angiography(OCTA)in three groups. The differences and correlations between the parameters were compared. <p>RESULTS: LLT(69.87±11.401nm)in normal control group was higher than that in NPDR(54.87±7.453nm)and PDR group(42.67±5.246nm), and FAZ(0.312±0.021mm2)was lower than that in NDPR group(0.389±0.037mm2)and PDR group(0.437±0.032mm2). The vascular density of SCL(51.977%±4.164%)was significantly higher than that of NPDR(47.067%±4.757%)and PDR(41.865%±5.512%), and that of DCL(49.578%±2.619%)was higher than that of NPDR(46.032%±2.622%)and PDR(40.598%±2.671%)(all <i>P</i><0.01). There was no correlation between LLT, FAZ, SCL and DCL in normal subjects. LLT was negatively correlated with FAZ in both NPDR group and PDR group(<i>r</i>=-0.922, <i>r</i>=-0.923, all <i>P</i><0.01), positively correlated with SCL(<i>r</i>=0.798, <i>r</i>=0.902, all <i>P</i><0.01), and had no correlation with DCL(<i>r</i>=0.140, <i>r</i>=0.073, <i>P</i>=0.285, <i>P</i>=0.581).<p>CONCLUSION: In diabetic retinopathy, the lipid layer of tear film is lower and the stability of tear film is decreased, and there is a correlation between diabetic dry eye and macular microvascular changes.
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@#AIM: To observe the effects of butterflybush flower eye drops at different concentrations on expression of inflammatory crtokines IL-1β, Mucin 5AC(MUC5AC)and P38MAPK in castrated male rabbits, and to explore the therapeutic effect of that drops on dry eyes. <p>METHODS: Thirty-six male rabbits were randomly divided into blank group(A), model group(B), low concentrations butterflybush flower eye drops group(C, 1mg/mL), the medium concentrations drops group(D, 1.5mg/mL), the high concentrations drops group(E, 3mg/mL), and testosterone group(F). In addition to group A, the testes and epididymis were removed from each group to establish a dry eye animal model. After successful modeling, groups A and B remain unchanged. Groups C, D, and E were given different concentrations of butterflybush flower eye drops, 3 times/d. In group F, testosterone propionate was injected into the muscles of the thigh at a dose of 0.5mL/kg once every 3d. Fluorescein staining, Schirmer I test(SⅠt)and tear film break time(BUT)were measured under general anesthesia in each group, eatment. After 4wk of treatment, the rabbits were sacrificed and the conjunctival tissues of the eyes were taken. The expression of IL-1β, mucin 5AC and P38MAPK in the conjunctiva was detected by immunohistochemical staining.<p>RESULTS: Among low concentrations butterflybush flower eye drops group, the medium concentrations drops group and the high concentrations drops group, the SⅠt value was significantly higher than that of model group, and BUT was significantly longer than model group. The positive staining of corneal fluorescein was significantly improved compared with model group, which was statistically significant(<i>P</i><0.01). Among IL-1β and P38MAPK in the conjunctiva of high concentrations butterflybush flower eye drops group, the medium concentrations drops group and the low concentrations drops group, the positive expressions were lower than those in model group, and the expression of MUC5AC was higher than that in group model group(<i>P</i><0.01). In addition, the high concentrations drops group was superior to the low and the medium concentrations drops group.<p>CONCLUSION: Butterflybush flower eye drops have androgen-like effect. For castrated dry eyes of male rabbits, they can down-regulate the expression of IL-1beta and P38MAPK in dry conjunctival tissue and increase the expression of MUC5AC, thus reducing inflammation infiltration in dry conjunctival tissue and maintaining tear film stability, but their effect is weaker than that of androgen. To the treatment of dry eyes, the middle and high concentration groups of the drops had stronger effects than the low one, and the high concentration group was better than the medium one.
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Introduction: Lack of sufficient production of tear filmleads to symptoms of dry eyes. Sleep deprivation is knownto cause various physiological changes in the body includinghormonal and neuronal changes. These changes can lead todisturbance in development of a proper tear film and hence dryeyes. Current research objective was to study the associationof sleep deprivation and occurrence of dry eyes in the sleepdeprived individuals.Material and methods: Medical students from a tertiarycare center were included in the study. Sleep deprivationwas estimated using Sleep-quality questionnaire (TheSleep Revolution by Arianna Huffington). Dry eyesquestionnaire was taken and the severity assessed usingOcular Surface Disease Index Scale (OSDI). Schirmer’s test,Tear Film Break-up Time (TBUT) and IOP measurementwere carried out on the test subjects. The results werecompared between sleep-deprived and non-sleep deprivedindividuals.Results: Out of total 50 test subjects, 25 were controls and25 were sleep deprived. Significant association was foundbetween sleep deprivation and severity of dry eye symptoms,tear film break up time and results of Schirmer’s tests.However, no significant association was found between sleepdeprivation and increase in IOP.Conclusion: Sleep deprivation induces reduction in tearsecretion; increase in tear osmolarity and shortens tear filmbreak-up time. These changes can later lead to developmentof ocular surface diseases. Hence, a larger study is neededto be carried out to further study the association andspread awareness regarding need of good quality sleep inorder to reduce occurrence of dry eyes and other oculardisorders.
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PURPOSE: To evaluate the efficacy of 3% diquafosol tetrasodium (DQ) after laser-assisted in-situ keratomileusis (LASIK). METHODS: This prospective randomized study included 150 eyes in 75 patients who were scheduled for LASIK. The patients in the 3% diquafosol tetrasodium (DQ) group (37 patients, 74 eyes) were instructed to apply one drop of DQ, six times daily post-op, while the patients in the 0.3% sodium hyaluronate (HA) group (38 patients, 76 eyes) were instructed to apply one drop of HA, six times daily post-op. A Schirmer test, tear film break-up time (BUT), corneal and conjunctival fluorescein staining score (FLSS), and ocular surface disease index (OSDI) were evaluated pre-op and at 1, 4, and 12 weeks post-op while the tear osmolarity was evaluated pre-op and at 4 and 12 weeks post-op. RESULTS: There was no significant difference between the two groups regarding Schirmer test results or tear osmolarity and conjunctival FLSS. The BUT was significantly higher in the DQ group at 1 week and 12 weeks post-op. The corneal FLSS was significantly lower in the DQ group at 1 week, 4 weeks and 12 weeks post-op. The OSDI was significantly lower in the DQ group at 1 week post-op. CONCLUSIONS: Use of 3% diquafosol tetrasodium after surgery improved ocular dryness and increased the tear film stability.
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Humans , Fluorescein , Hyaluronic Acid , Keratomileusis, Laser In Situ , Osmolar Concentration , Prospective Studies , TearsABSTRACT
@#AIM:To observe and compare the therapeutic effects of intense pulsed light(IPL)combined with meibomian gland massage and eyelid fumigation massage on dry eyes associated with meibomian gland dysfunction(MGD).<p>METHODS: This study was a prospective randomized controlled clinical trial. Totally 73(146 eyes)outpatients with MGD-related dry eyes were selected from March 2018 to August 2018 at Xi'an No.4 Hospital. 38 patients(76 eyes)in the experimental group were given IPL combined meibomian gland massage treatment(once every three weeks, three times in all), and 35 patients(70 eyes)in the control group were given eyelid fumigation combined meibomian gland massage treatment(fumigating and cleaning the eyelid margin every day for 5d, massaging the tarsal gland on the fifth day, repeating treatment after 2wk, totally 3 times). The study lasted 12wk. The ocular surface disease index(OSDI), standard dry eye assessment questionnaire(SPEED), non-invasive tear film rupture time(NITBUT), non-invasive lacrimal river height measurement(NITMH), meibomian gland loss score(MGS), meibomian gland secretion assessment(MGYSS)before and after the first treatment, the first week, the fourth week, the seventh week and the twelfth week were recorded to evaluate the curative effect of the two treatments.<p>RESULTS: There was no significant difference in gender and age between the two groups(<i>P</i>>0.05).There was no significant difference in data between the two groups before treatment(<i>P</i>>0.05). Indexes except NITMH and MGS each time point after treatment in the two groups were significantly improved compared with those before treatment, and the difference was statistically significant(<i>P</i><0.05). There was no significant difference in each index between the experimental group and the control group at 1wk after treatment(<i>P</i>>0.05); at 4, 7 and 12wk after treatment, the experimental group was superior to the control group except for NITMH and MGS, and the difference was statistically significant(<i>P</i><0.05). From the change trend of the indicators, the therapeutic effect of the experimental group continued to improve after the first treatment, and was the best at the 12th week, while that of the control group was the best at the 7th week after the first treatment, and then the curative effect was weakened. There were no obvious complications in both groups after treatment.<p>CONCLUSION: Both intense pulsed light combined with palpebral gland massage and eyelid fumigation massage are convenient, safe and effective for MGD-related dry eyes, and the curative effect and maintenance time of intense pulsed light combined with palpebral gland massage are better than that of eyelid fumigation massage.
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Objective To synthesize a dual-function nanodrug with immunosuppression of tacrolimus (FK506) and lubrication of artificial tear carboxymethylcellulose sodium (CMC), and provide a basis for the clinical treatment of dry eye syndrome. Methods The FK506 nanocrystals were prepared by ultrasonic method, and polyallylamine hydrochloride (PAH) and CMC were deposited alternatively on the surface of FK506 nanocrystals using layer-by-layer (LbL) self-assembly technology to prepare the bifunctional nanodrug. The morphology, particle size, surface charge, and composition of the nanodrug were analyzed. Results The particle size of FK506 nanocrystals was uniform, and the FK506-(PAH/CMC)3 was approximately spherical with uneven surface. Zeta potential detection results showed that the charge changed alternatively with the increasing of layer number. The results of laser scanning confocal imaging and infrared spectroscopy showed that PAH and CMC were successfully modified on the surface of FK506 nanocrystals. Conclusion The prepared FK506-(PAH/CMC)3 nanodrug is expected to provide a scientific basis for the combined treatment of dry eye.
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· AIM:To investigate the prevalence and related factors of dry eye in primary school pupils in Lanzhou,Gansu Province.· METHODS:From October to November 2016,1347 pupils in two primary schools in Lanzhou,Gansu Province,were randomly selected as subjects.Every pupil was carried on the questionnaire of dry eye and eye inspection to confirm the diagnosis of dry eye.Besides,the prevalence and influencing factors of dry eye in pupils were analyzed by chi-square test,Mann-Whitney U test and logistic regression model.· RESULTS:A total of 1 268 pupils took part in this study and the inclusion ratio was 94.14％;271 individuals were diagnosed as dry eye,and the prevalence rate was 21.37％.Statistical analysis showed that the risk factors of dry eye were male,senior pupils,often using eye drops,poor reading habits,wearing contact lenses,video terminals last for a long time,learning pressure.· CONCLUSION:Dry eye has become one of the main diseases that plagued pupil's life and learning.It should cause wide attention.Considering the above factors,rational use of eye and improve lifestyle will help to reduce the damage to eye of pupils.
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· AIM:To investigate the efficacy and safety of short-term application of pranoprofen eye drops in the treatment of dry eyes in senile patients.· METHODS:Totally 100 elderly patients with dry eyes treated in our hospital from June 2016 to May 2017 were randomly divided into 2 groups.All cases were bilateral onset,and the right eyes were marked as the observation eyes.The 50 eyes in the observation group were treated with 1 g/L pranoprofen eye drops combined with 1 g/L sodium hyaluronate eye drops,while 50 in the control group were treated with 1 g/L sodium hyaluronate eye drops.The results of Schirmer Ⅰ,ocular surface disease index (OSDI),break-up time (BUT),ocular surface staining(OSS),human leukocyte antigen-DR (HLA-DR) and CD11b in conjunctival epithelial cells before and at 2wk after treatment,and adverse reactions in 2 groups were observed.· RESULTS:Before treatment,results of Schirmer I,OSDI,BUT,OSS,HLA-DR and CD11b in the two groups were similar,there were no statistically significant differences (P＞0.05).After treatment,OSDI,OSS,HLA-DR,CD11b significantly decreased,BUT significantly increased in both groups,the differences were statistically significant (P＜ 0.05).After treatment,OSDI,OSS and HLA-DR in the observation group were significantly lower than those in the control group,BUT in the observation group was significantly higher than those in the control group,the differences were statistically significant (P＜ 0.01).The expression of HLA-DR was positively correlated with OSDI and OSS,and negatively correlated with BUT,and the correlations were statistically significant (P＜0.06).· CONCLUSION:Short term application of pranoprofen eye drops can effectively enhance the efficacy in treating elderly patients with dry eyes,release clinical symptoms,and the therapeutic mechanism may be related to the inhibitory effect on HLA-DR.
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Objetivo: describir el comportamiento del síndrome de ojo seco en pacientes diabéticos de un área de salud. Métodos: se realizó un estudio observacional descriptivo transversal. La muestra la conformaron 134 pacientes diabéticos que asistieron a la consulta del Policlínico Mario Muñoz, de septiembre de 2013 a febrero de 2014. Se estudiaron variables sociodemográficas, así como síntomas, signos y gravedad del ojo seco. Se aplicaron métodos de estadística descriptiva para variables cualitativas. Para el procesamiento y análisis de la información se confeccionó una base de datos en el programa estadístico Microsoft Excel 2003. Resultados: el 88,8 por ciento de los pacientes tenían más de 50 años. Predominó el grupo de 60-69 años y el sexo femenino a razón de 97:37 por cada 2,6 mujeres diagnosticadas con diabetes mellitus. Se diagnosticó un hombre. Predominó la diabetes mellitus tipo 2 (97,8 por ciento) y de ellos 74,7 por ciento presentaron ojo seco. También predominaron los pacientes con sintomatología de ojo seco (38 por ciento) y menos de 5 años de evolución de la diabetes (57,4 por ciento). La visión borrosa resultó el síntoma más frecuente (62,7 por ciento) y la inyección conjuntival el signo (54,9 por ciento). El ojo seco leve predominó en el 98 por ciento de los casos. Conclusiones: la diabetes mellitus es un problema de salud real. Las personas diabéticas presentan con frecuencia daño de la superficie ocular y síndrome de ojo seco, por lo que deben visitar al oftalmólogo periódicamente(AU)
Objective: to describe the behavior of the dry eye syndrome in diabetic patients from a health area. Method: a cross-sectional, descriptive and observational study was carried out. The sample was made up by 134 diabetic patients who had been seen at "Mario Muñoz" Policlinics from September 2013 to February 2014. Sociodemographic variables as well as symptoms, signs and severity of the dry eye syndrome were all studied. Summary statistic methods were applied in qualitative variables. For data processing and information, a database according to Microsoft Excel statistical program 2003 was developed. Results: in the sample, 88.8 percent of the patients were older than 50 years, prevailing the 60-69 year-old group and females at a ratio of 97:37, that is, 2.6 women were diagnosed with diabetes mellitus per one man. Diabetes mellitus type 2 prevailed (97.8 percent) and from this figure, 74.7 percent presented with dry eye. Likewise, patients with dry eye symptoms (38 percent) and less than 5 years of diabetes progression (57.4 percent) were also predominant. Blurred vision was the most frequent symptom (62.7 percent) and the conjunctival injection the most common sign (54.9 percent). Mild dry eye syndrome prevailed in 98 percent of cases. Conclusions: diabetes mellitus is a real health problem. Diabetic people frequently present damage of the ocular surface and dry eye syndrome, therefore, they should go systematically to the ophthalmologist's(AU)
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Humans , Female , Middle Aged , Aged , Data Interpretation, Statistical , Diabetes Mellitus/epidemiology , Dry Eye Syndromes/diagnosis , Cross-Sectional Studies , Epidemiology, Descriptive , Observational StudyABSTRACT
·Corneal confocal microscopy can be used in the real-time, noninvasive, high-resolution corneal structure at the cellular level, which has been widely used in corneal disease research. This review summarizes recent advances in corneal confocal microscopy in the study of infectious keratitis, dry eye, keratoconus, diabetic peripheral neuropathy, and clinical studies to guide corneal transplantation.
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·AIM: To compare and analyze the clinical efficacy of different artificial tears for patients with xerophthalmia after phacoemulsification.·METHODS:Retrospective analysis on the clinical data of 150 cases (150 eyes) treated by phacoemulsification in our hospital from March 2014 to April 2017. And according to the artificial tears used, they were divided into Group A ( control group) and B ( application of sodium hyaluronate eye drops) , Group C ( used carbomer gel eye drops) ,50 cases 50 eyes in every group. We compared and analyzed the BUT, FL, SⅠt findings, OSDI scores preoperatively and 1wk, 1 and 3mo postoperatively, and visual acuity before and 3mo after operation in the three groups.·RESULTS:There were no differences in BUT, FL, SⅠt and OSDI scores between the three groups before and at 1wk after the operation (P>0. 05). At 1mo after operation, the levels of BUT and SⅠt in Group B and Group C were higher than those in Group A, and the scores of OSDI and FL were lower than those in Group A (P<0. 05); between Group B and Group C there was no difference in BUT, FL, SⅠt levels and OSDI score (P>0. 05). After 3mo, the levels of BUT and SⅠt in the Group B and Group C were higher than that in the Group A, and the FL level and OSDI score were lower than those in the Group A ( P<0. 05);the levels of BUT and SⅠt in the Group C were higher than those in the Group B, and the FL level and the OSDI score were lower than those in the Group B ( P<0. 05 ) . The visual acuity of the three groups was improved at 3mo after the operation (P<0. 05), and there was no difference in LogMAR visual acuity between the three groups before and 3mo after operation (P>0. 05).· CONCLUSION: different types of artificial tear can improve the symptoms of dry eyes in patients age-related cataract after phacoemulsification, in which carbomer eye drops or lipid containing artificial tears improve postoperative dry eye symptoms and signs, and will not affect the recovery of visual acuity.
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@#AIM:To investigate the relationship between dry eyes incidence and poor vision in primary and middle school students in the urban-rural fringe of Guangzhou. <p>METHODS: We used stratified cluster random sampling, extracted the urban-rural fringe elementary school and middle school, gave out self-made questionnaire survey to 3572 students, at the same time, underwent ophthalmology routine inspection and tear secretion test, rupture time measurement and corneal and conjunctival fluorescein staining. <p>RESULTS: The overall number of poor vision was 764 people(1447 eyes), the overall poor vision was 20.25% counted by eye amount, the rate of poor vision in primary school was 14.08%, in junior high school was 29.51%, in junior middle school was significantly higher than that in primary school(<i>P</i><0.05). The number of subjects with dry eyes was 424 cases(848 eyes), the overall incidence of dry eyes was 11.87%, among them the occurring rate of dry eyes in primary school was 5.14%, in junior middle school was 21.96%, which was significantly higher than that in primary school(<i>P</i><0.05). Ordered multivariate Logistic regression analysis showed that junior high school, doing homework for 1-3h or >3h every day, eyes away from the book <30cm, lying down when reading books, watching TV or playing computer phone for 1-3h and >3h every day, dry eyes, and the nutritional status(fat or thin)were the risk factors of poor vision in primary and middle school students the(<i>OR</i>=3.862, 1.164, 1.210, 1.212, 1.315, 1.319, 3.040, 1.110, 1.057 and 1.141). <p>CONCLUSION: The risk factors of primary and middle school students vision is multifarious, dry eyes is a risk factor for poor vision of primary and middle school students in the urban-rural fringe of Guangzhou.
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Background Reasearches showed that α-melanocyte-stimulating hormone (α-MSH) inhibits inflammation and ameliorates the ocular surface abnormalities in a scopolamine-induced dry eye rat model,and the managing effect of sodium carboxymethylcellulose (CMC) on dry eyes also has been determined.However,whether α-MSH can enhance the therapeutic effects of CMC remains to be investigated.Objective This study was to investigate the protective effects of α-MSH combined with CMC on ocular surface in a scopolamine-induced dry eye rat model.Methods Sixty clean female Wistar rats were randomly divided into normal control group,model control group,NaCl group,CMC group,α-MSH group and α-MSH+CMC group,and 10 rats for each group.The dry eye models were established by subcutaneous injection of scopolamine hydrobromide at 9:00,12:00,15:00 and 18:00 per day for 28 days.0.9％ NaCl solution,1×10 3 mg/ml α-MSH solution,0.5％ CMC eye drop,and 1 ×10-3 mg/ml α-MSH+0.5％ CMC solution were topically administered twice a day (8:00,17:00) since the initial day of modeling according to grouping.Shirmer Ⅰ test (S Ⅰ t),breakup time of tear film (BUT) and corneal fluorescence staining were performed before and 7,14,21,28 days after the application of drugs.At 28 days following the administration of drugs,the eyeballs of the rats were collected.Hemotoxylin and eosin staining was employed to examine the morphology of corneas,and periodic acid schiff (PAS) staining was used to count the conjunctival goblet cells.This study protocol was approved by Experimental Animal Ethic Committee of Tianjin Medical University (SYXK 2009-0001),and the use and care of the rats complied with ARVO Statement.Results The S Ⅰ t and BUT values were significantly reduced,and the corneal fluorescence staining scores were significantly increased over time following modeling in the model control group (all at P＜0.01).No significant differences were found in the S Ⅰ t,BUT and corneal fluorescence staining scores between model control group and NaCl group at various time points (all at P＞0.05).At 7,14 and 21 days after intervention,the S Ⅰ t values were (4.800±0.789),(4.100±0.516) and (4.300±0.856) mm in the α-MSH+CMC group,which were considerably higher than (2.875 ±0.719),(2.375 ±0.619) and (2.532±0.957)mm in the NaCl group (all at P＜0.01).At 7 days after intervention,the BUT values were (4.938± 1.843) seconds and (5.000±1.491) seconds in the α-MSH group and α-MSH+CMC group,which were significantly higher than (3.250±1.000) seconds in the NaCl group (both at P＜0.01).The corneal fluorescence staining scores in the CMC group,α-MSH group and α-MSH+CMC group were significantly lower than that in the NaCl group,with the lowest score in the α-MSH +CMC group (all at P＜0.05).The thickening of corneal epithelial layer,corneal edema and arrangement disorder of corneal stroma were found in the model control group and NaCl group;while slight corneal edema and epithelial cell proliferation were exhibited in the α-MSH+CMC group by hemotoxylin and eosin staining.PAS staining showed that the number of goblet cells was much more in the CMC group,α-MSH group and α-MSH+ CMC group than that in the model control group and NaCl group (all at P ＜ 0.01).Conclusions The sole application of α-MSH or CMC alleviates ocular surface damage and morphological abnormality to certain extent,and the combination of α-MSH and CMC generates more effective protection in comparison with sole administration of α-MSH or CMC.The early application of the drugs plays an improvement role in tear secretion and tear film stability in dry eyes.
ABSTRACT
Background Studies showed that inflammation is associated with the pathogenesis and development of dry eyes,and tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) are key inflammatory factors.Thymosin β4 (Tβ4) plays a promoting effect on the migration of epithelial cells and anti-inflammatory action.However,the influences of Tβ4 on the repair of ocular surface in dry eyes are unelucidated.Objective This study was to investigate the regulation of Tβ4 to the expressions of TNF-o and IFN-γand its effect on the recovery of ocular surface in rat dry eye models.Methods The dry eye models were induced by topically administered of benzalkonium chloride (BAC) for consecutive 7 days in the left eyes of 50 SPF male SD rats,and 36 successful models were used in the experiment.Tβ4 solution (9 μg/ml),recombinant human epithelial growth factor (rhEGF)and sterile PBS at 5 μl was topically administered three times for consecutive 7 days in the Tβ4 group,rhEGF group and PBS group,and no drug was used in the model control group.The normal right eyes of rats served as the normal control group.The break-up time of tear film (BUT),corneal fluorescein staining score and Schirmer Ⅰ test (S I t)were examined and evaluated in the rats on the seventh day after administration of drugs.Then the rats were sacrificed by excessive anesthesia and the sections of the ocular surface were prepared.The morphology of the specimens was examined by hematoxylin and eosin staining,and the number of conjunctival gobelt cells was counted by periodic acidschiff staining.The ultrastructure of the corneal and conjunctival cells was examined under the transmission electron microscope.The expressions of TNF-α mRNA and IFN-γ mRNA and their proteins in conjunctiva tissue were quantified by quantitative real-time PCR and Western blot,respectively.The use and care of the animals followed by Regulation for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission.Results The BUT was (10.42±0.66),(7.46±0.49),(8.71±0.50),(9.59±0.35) and (8.63± 0.68) seconds in the normal control group,model control group,rhEGF group,Tβ4 group and BUT group,showing a significant difference among the groups (F =5.65,P =0.00),and the BUT was evidently shortened in the model control group compared with the normal control group,while the BUT was significantly extended in the rhEGF group and Tβ4 group in comparison with the model control group (all at P＜0.05).No significant differences were found in the corneal fluorescence score and S I t among the groups (F =0.42,P =0.79;F =136.77,P =0.00).The corneal and conjunctival epithelium defect and corneal stromal edema were seen in the model control group,and the proliferation of the epithelial cells were found in the rhEGF group and Tβ4 group,with the irregulated arrangement of the cells.A considerable difference was seen in the number of conjunetival goblet cells among the groups (F=3.16,P =0.04),and the number of eonjunctival goblet cells in the rhEGF group and model control group was significantly less than that in the normal control group (all at P＜0.05),and no statistically significant difference was seen between Tβ4 group and normal control group (P ＞ 0.05).The swelling,mergence,crispation,rupture and decrease of the microvilli and micro fold were found in the model control group,and the repair of the cell microvilli was seen in the Tβ4 group.The expressions of the TNF-α mRNA,IFN-γ mRNA and their proteins in the conjunctiva were significantly different among the groups (F =43.08,371.69,34.27,43.52,all at P =0.00),the expressions of the inflammatory factors were significantly higher in the model control group compared with the normal control group,and these expressions were evidently lower in the Tβ4 group in comparison with the model control group and rhEGF group (all at P＜0.05).Conclusions The topical administration of Tβ4 solution can promote the repair of ocular surface by down-regulating the expression of TNF-α and IFN-γ in conjunctiva and stablize the tear film in rat dry eyes.
ABSTRACT
El ordenador, si no se utiliza adecuadamente, produce trastornos en la salud. La causa de estas alteraciones está relacionada con factores ergonómicos visuales del entorno de trabajo y la exacerbación de problemas visuales ya existentes. Objetivo: describir características clínicas del síndrome de visión de la computadora en trabajadores de dos bancos metropolitanos de un área de salud. Métodos: se realizó un estudio descriptivo transversal. La muestra la conformaron 40 trabajadores que refirieron sintomatología asociada al uso del ordenador y que cumplieron los criterios de inclusión. Se estudiaron las variables: edad, sexo, síntomas, signos biomicroscópicos, afecciones oculares asociadas y tipo de defecto refractivo. Resultados: el 82,5 por ciento de los pacientes tenían más de 30 años, con edad media de 41,7 años. Por cada 12,3 mujeres diagnosticadas con síndrome de visión de la computadora se diagnosticó un hombre. Ojo rojo intermitente y visión borrosa resultaron los síntomas más frecuente y la inyección conjuntival el signo. Las afecciones oculares más frecuentes fueron las ametropías con 87,5 por ciento y ojo seco con 57,5 por ciento. Dentro de las ametropías predominó el astigmatismo con 43 por ciento de casos. Conclusiones: el síndrome de visión de la computadora es un problema de salud real en la actualidad. Las personas que pasan muchas horas frente al ordenador generalmente presentan antecedentes de afecciones oculares como ametropías y ojo seco, por lo que deben asistir al oftalmólogo(AU)
The computer, if not properly used, may cause health disorders. The cause is already related with visual ergonomic factors of the work environment and the exacerbation of already existing visual problems. Objective: to describe the clinical characteristics of the computer vision syndrome in employees from two metropolitan banks in a health area. Method: a cross-sectional descriptive study was carried out. The sample made up of 40 workers who had symptoms associated to the use of computer and met the inclusion criteria. The study variables were age, sex, symptoms, biomicroscopy signs, other eye pathologies and type of refractive defect. Results: in the study group, 82,5 percent of the patients were older than 30 years old, being the mean age of 41,7 years. One man per 12,3 women was diagnosed with the computer vision syndrome. Intermittent red eye and blurred vision were the most frequent symptoms and the conjunctival injection was the sign. The most frequent ocular pathologies turned to be the ametropia with 87,5 percent and dry eye with 57,5 percent of cases. Astigmatism prevailed in 43 percent of the group. Conclusions: the computer vision syndrome is a real health problem at the present time people that spend many hours working with computer generally show eye alterations as ametropia and dry eye, and they should go to the ophthalmologist(AU)